Владислава Ковальська, д.б.н.

Патенти:

  1. Pat. WO2011/065980 (A2), 03.06.2011. Dyes for Analysis of Protein Aggregation.  Patton WF; Yarmoluk SM; Pande P; Kovalska V; Dai L; Volkova K; Coleman L; Losytskyy M; Ludlam A; Balanda A; Shen D.
  2. Patent Application US2008091015 A1, 17.04.2008; WO 2008/027821 A1, 06.03.2008. Use of squaraine dyes to visualize protein during separations. Berkelman TR, Yarmoluk SM, Kovalska VB, Losytskyy MYu, Volkova KD.
  3. Patent Application US2006207881, 21.09.2006. Detection of polyamino acids using trimethincyanine dyes. Kovalska VB; Kryvorotenko DV; Losytskyy MYu; Nording P; Rueck A; Schoenenberger B; Yarmoluk SM; Wahl F.

Публікації:

  1. Kuperman M, Losytskyy M, Bykov A, Yarmoluk S, Zhizhin K, Kuznetsov N, Varzatskii O, Gumienna-Kontecka E, Kovalska V (2017) Effective binding of perhalogenated closo-borates to serum albumins revealed by spectroscopic and ITC studies Journal of Molecular Structure 1141:75-80
  2. Blechinger J, Varzackii O, Kovalska V, Zelinskiid G, Voloshin Ya, Kinski E, Mokhir A (2016) Cytotoxicity of electrophilic iron(II)–clathrochelates in human promyelocytic leukemia cell line. Bioorganic & Medicinal Chemistry Letters 26:626-629
  3. Kovalska V, Chernii S, Losytskyy M, Dovbii Y, Tretyakova I, Czerwieniec R, Chernii V, Yarmoluk S, Volkov S (2016) b-ketoenole dyes: synthesis and study as fluorescent sensors for protein amyloid aggregates. Dyes and Pigments 132:274-281.
  4. Losytskyy MYu, Kovalska VB, Varzatskii OA, Kuperman MV, Potocki S, Gumienna-Kontecka E, Zhdanov AP, Yarmoluk SM, Voloshin YZ, Zhizhin KYu, Kuznetsov NT, Elskaya AV (2016) An interaction of the functionalized closo-borates with albumins: The protein fluorescence quenching and calorimetry study. J Luminesc 169:51-60.
  5. Kuperman MV, Chernii SV, Losytskyy MYu, Kryvorotenko DV, Derevyanko NO, Slominskii YuL, Kovalska VB, Yarmoluk SM (2015) Trimethine cyanine dyes as fluorescent probes for amyloid fibrils: The effect of N,N'-substituents. Analytical Biochemistry 484:9-17.
  6. Kovalska VB, Losytskyy MY, Varzatskii OA, Cherepanov VV, Voloshin YZ, Mokhir AA, Yarmoluk SM, Volkov SV (2014) Study of anti-fibrillogenic activity of iron(II) clathrochelates. Bioorg Med Chem 22(6):1883-1888.
  7. Inshyn DI, Kovalska VB, Losytskyy MY, Slominskii YL, Tolmachev OI, Yarmoluk SM (2014) Development of a quantitative structure activity relations (QSAR) model to guide the design of fluorescent dyes for detecting amyloid fibrils. Biotech and Histochem 89(1):1-7.
  8. Kovalska VB, Losytskyy MYu, Reis LV, Santos PF, Yarmoluk SM (2014) Fluorescent detection of a partially unfolded conformation of beta-lactoglobulin using squaraine dyes. Macromol Symp 335(1):43-50.
  9. Kovalska V, Cherepanov V, Losytskyy M, Chernii S, Senenko A, Chernii V, Tretyakova I, Yarmoluk S, Volkov S (2014) Anti-fibrillogenic properties of phthalocyanines: Effect of the out-of-plane ligands. Bioorg Med Chem 22(24):6918-6923. 
  10. Kovalska VB, Losytskyy MY, Chernii SV, Chernii VY, Tretyakova IM, Yarmoluk SM, Volkov SV (2013) Towards the anti-fibrillogenic activity of phthalocyanines with out-of-plane ligands: Correlation with self-association proneness. Biopolym Cell 29(6):473-479.
  11. Losytskyy MY, Kovalska VB, Varzatskii OA, Sergeev AM, Yarmoluk SM, Voloshin YZ (2013) Interaction of the iron(II) cage complexes with proteins: Protein fluorescence quenching study. J Fluoresc 23(5):889-895.
  12. Kovalska VB, Losytskyy MY, Tolmachev OI, Slominskii YL, Segers-Nolten GMJ, Subramaniam V, Yarmoluk SM (2012) Tri- and pentamethine cyanine dyes for fluorescent detection of α-synuclein oligomeric aggregates. J Fluoresc 22(6):1441-1448.
  13. Kovalska V, Losytskyy M, Chernii V, Volkova K, Tretyakova I, Cherepanov V, Yarmoluk S, Volkov S (2012) Studies of anti-fibrillogenic activity of phthalocyanines of zirconium containing out-of-plane ligands Bioorg Med Chem 20(1):330-334.
  14. Volkova KD, Kovalska VB, Losytskyy MYu, Reis LV, Santos PF, Almeida P, Lynch DE, Yarmoluk SM (2011) Aza-substituted squaraines for the fluorescent detection of albumins. Dyes and Pigments 90:41-47.
  15. Kovalska VB, Losytskyy MYu, Yarmoluk SM, Lubitz I, Kotlyar AB (2011) Mono and trimethine cyanines cyan 40 and Cyan 2 as probes for highly selective fluorescent detection of non-canonical DNA structures. J Fluoresc 21(1):223-230.
  16. Volkova KD, Kovalska VB, Inshin D, Slominskii YL, Tolmachev OI, Yarmoluk SM (2011) Novel fluorescent trimethine cyanine dye 7519 for amyloid fibril inhibition assay. Biotech Histochem 86(3):188-191.
  17. Kovalska VB, Losytskyy MYu, Lubitz I, Kotlyar AB, Yarmoluk SM (2010) Mono and trimethine cyanines Cyan 40 and Cyan 2 as probes for highly selective fluorescent detection of non-canonical DNA structures. J Fluoresc 21(1):223-230.
  18. Kovalska VB, Volkova KD, Manaev AV, Losytskyy MYu, Okhrimenko IN, Traven VF, Yarmoluk SM (2010) 2-Quinoline and coumarin polymethines for the detection of proteins using fluorescence. Dyes and Pigments 84:159-164.
  19. Tokar VP, Losytskyy MYu, Ohulchanskyy TY, Kryvorotenko DV, Kovalska VB, Balanda AO, Dmytruk IM, Yashchuk VM, Yarmoluk SM (2010) Styryl dyes as two-photon excited fluorescent probes for DNA detection and two-photon laser scanning fluorescence microscopy of living cells. J Fluoresc 20(4):865-872.
  20. Volkova KD, Kovalska VB, Balanda AO, Slominskii YuL, Tolmachev OI, Subramaniam V, Yarmoluk SM (2008) Studies of mono- and trimethine cyanine dyes as specific fluorescent probes for fibrillarβ-lactoglobulin detection. Ukrainica Bioorganica Acta 6(2):15-21.
  21. Volkova KD, Kovalska VB, Segers-Nolten GM, Veldhuis G, Subramaniam V, Yarmoluk SM (2008) Explorations of the application of cyanine dyes for quantitative alpha-synuclein detection. Biotechnic & Histochemistry 84(2):55-61.
  22. Volkova KD, Kovalska VB, Losytskyy MYu, Bento A, Reis LV, Santos PF, Almeida P, Yarmoluk SM (2008) Studies of benzothiazole and benzoselenazole squaraines as fluorescent probes for albumins detection.Journal of Fluorescence 18(5):877-882.
  23. Yarmoluk SM, Kovalska VB, Losytskyy My (2008) Symmetric cyanine dyes for detecting nucleic acids.Biotechnic and Histochemistry 83(3-4):131-145.
  24. Volkova KD, Kovalska VB, Balanda AO, Losytskyy MYu, Golub AG, Vermeij RJ, Subramaniam V, Tolmachev OI, Yarmoluk SM (2008) Specific fluorescent detection of fibrillar α-synuclein using mono- and trimethine cyanine dyes. Bioorganic and Medicinal Chemistry 16(3):1452-1459.
  25. Akbay N, Losytskyy MYu, Kovalska VB, Balanda AO, Yarmoluk SM (2008) The mechanism of benzothiazole styrylcyanine dyes binding with dsDNA: studies by spectral-luminescent methods. Journal of Fluorescence 18(1):139-147.
  26. Volkova KD, Kovalska VB, Yarmoluk SM (2007) Modern techniques for protein detection on polyacrylamide gels: problems arising from the use of dyes of undisclosed structures for scientific purposes. Biotechnic and Histochemistry 82(4&5):201-208.
  27. Volkova KD, Kovalska VB, Balanda AO, Vermeij RJ, Subramaniam V, Slominskii YuL, Yarmoluk SM (2007) Cyanine dye-protein interactions: looking for fluorescent probes for amyloid structures. Journal of Biochemical and Biophysical Methods 70(5):727-733.
  28. Xu C, Losytskyy MYu, Kovalska VB, Kryvorotenko DV, Yarmoluk SM, McClelland S, Bianco PR (2007) Novel, monomeric cyanine dyes as reporters for DNA helicase activity. Journal of Fluorescence 17(6):671-685.
  29. Yashchuk VM, Gusak VV, Dmytruk IM, Prokopets VM, Kudrya VYu, Losytskyy MYu, Tokar VP, Gumenyuk YaO, Yarmoluk SM, Kovalska VB, Balanda AO, Kryvorotenko DV (2007) Two-photon excited luminescent styryl dyes as probes for the DNA detection and imaging. Photostability and phototoxic influence on DNA. Molecular Crystals and Liquid Crystals 467(1):325-338.
  30. Yashchuk VM, Kudrya VYu, Losytskyy MYu, Tokar VP, Yarmoluk SM, Dmytruk IM, Prokopets VM, Kovalska VB, Balanda AO, Kryvorotenko DV, Ogul'chansky TYu (2007) The optical biomedical sensors for DNA detection and imaging based on two-photon excited luminescent styryl dyes: phototoxic influence on the DNA.SPIE 6796
  31. Losytskyy MYu, Akbay N, Kovalska VB, Balanda AO, Boutorine A, Yarmoluk SM (2007) To the studies of (p-dimethylaminostyryl)pyridinium based homodimer to dsDNA binding mechanism. Ukrainica Bioorganica Acta 5(2):52-55.
  32. Balanda AO, Volkova KD, Kovalska VB, Losytskyy MYu, Tokar VP, Prokopets VM, Yarmoluk SM (2007) Synthesis and spectral-luminescent studies of novel 4-oxo-4,6,7,8-tetrahydropyrrolo[1,2-a]thieno[2,3-d]pyrimidinium styryls as fluorescent dyes for biomolecules detection. Dyes and Pigments 75(1):25-31.
  33. Volkova KD, Kovalska VB, Tatarets AL, Patsenker LD, Kryvorotenko DV, Yarmoluk SM (2007) Spectroscopic study of squaraines as protein-sensitive fluorescent dyes. Dyes and Pigments 72(3):285-292.
  34. Balanda AO, Volkova KD, Kovalska VB, Tokar VP, Yarmoluk SM (2006) Synthesis and spectral studies of styrylcyanines based on 4-oxo-4,6,7,8-tetrahydropyrrolo[1,2-a]thieno[2,3-d]pyrimidinium and 5-oxo-1,2,3,5-tetrahydropyrrolo[2,1-b]quinazolinium. Ukrainica Bioorganica Acta 4(2):10-15.
  35. Kovalska VB, Tokar VP, Losytskyy MYu, Deligeorgiev T, Vassilev A, Gadjev N, Drexhage K-H, Yarmoluk SM (2006) Studies of monomeric and homodimeric oxazolo[4,5-b]pyridinium cyanine dyes as fluorescent probes for nucleic acids visualization. Journal of Biochemical and Biophysical Methods 68(3):155-165.
  36. Kovalska VB, Volkova KD, Losytskyy MYu, Tolmachev OI, Balanda AO, Yarmoluk SM (2006) 6,6'-Disubstituted benzothiazole trimethine cyanines – new fluorescent dyes for DNA detection. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 65(2):271-277.
  37. Tokar VP, Losytskyy MYu, Kovalska VB, Kryvorotenko DV, Balanda AO, Prokopets VM, Galak MP, Dmytruk IM, Yashchuk VM, Yarmoluk SM (2006) Fluorescence of styryl dyes-DNA complexes induced by single- and two-photon excitation. Journal of Fluorescence 16(6):783-791.
  38. Kostenko OM, Kovalska VB, Volkova KD, Shaytanov P, Kocheshev IO, Slominskiy YL, Pisareva IV, Yarmoluk SM (2006) New method for covalent fluorescent biomolecule labeling with hemicyanine dyeю J. Fluoresc. 16(4): 589-593.
  39. Balanda AO, Volkova KD, Kovalska VB, Losytskyy MYu, Lukashov SS, Yarmoluk SM (2006) Novel styrylcyanines and their dimers as fluorescent dyes for nucleic acids detection: synthesis and spectral-luminescent studies. Ukrainica Bioorganica Acta 4(1):17-29.
  40. Solovyov OO, Livshits LA, Kovalska VB, Losytskyy MYu, Slominski YuL, Yarmoluk SM (2006) New highly sensitive fluorescent dye Barva NA for DNA visualization after the denaturing gradient gel electrophoresis. Ukrainica Bioorganica Acta 4(1):30-33.
  41. Kovalska VB, Losytskyy MYu, Kryvorotenko DV, Balanda AO, Tokar VP, Yarmoluk SM (2006) Synthesis of novel fluorescent styryl dyes based on the imidazo[1,2-a]pyridinium chromophore and their spectral-fluorescent properties in the presence of nucleic acids and proteins. Dyes and Pigments 68(1):39-45.
  42. Losytskyy MYu, Volkova KD, Kovalska VB, Makovenko IE, Slominskii YuL, Tolmachev OI, Yarmoluk SM (2005) Fluorescent properties of pentamethine cyanine dyes with cyclopentene and cyclohexene group in presence of biological molecules. Journal of Fluorescence 15(6):849-857.
  43. Kovalska VB, Kocheshev IO, Kryvorotenko DV, Balanda A, Yarmoluk SM (2005) Studies on the spectral-luminescent properties of the novel homodimer styryl dyes in complexes with DNA. Journal of Fluorescence 15(3):215-219.
  44. Matselyukh BP, Matselyukh DYa, Kovalska VB, Volkova KD, Kryvorotenko DV, Yarmoluk SM (2005) Studies of mutagenic activity of fluorescent DNA-sensitive monomethinecyanine and carbocyanine dyes in Ames test. Ukrainica Bioorganica Acta 3(2):27-34.
  45. Kovalska VB, Kryvorotenko DV, Balanda AO, Losytskyy MYu, Tokar VP, Yarmoluk SM (2005) Fluorescent homodimer styrylcyanines:synthesis and spectral-luminiscent stidies in nucleic acids and protein complex. Dyes and Pigments 67(1):47-54.
  46. Kovalska VB, Losytskyy My, Yarmoluk SM (2004) Luminescence spectroscopic studies of trimethinecyanines substituted in polymethine chain with nucleic acids and proteins. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 60(1-2):129-136.
  47. Matselyukh BP, Yarmoluk SM, Matselyukh AB, Kovalska VB, Kocheshev IO, Kryvorotenko DV, Lukashov SS (2003) Interaction of cyanine dyes with nucleic acids: XXXI. Using of polymethine cyanine dyes for the visualization of DNA in agarose gels. Journal of Biochemical and Biophysical Methods 57(1):35-43.
  48. Kovalska VB, Valyukh IV, Kostenko OM, Dmitrieva SYu, Tolmachov OI, Yarmoluk SM (2002) Interaction of cyanine dyes with nucleic acids. Spectral-luminescent properties of pyrilium and pyridinium mono/trimethincyanines. Biopolymers and Cell 16(6):540-546.
  49. Yarmoluk SM, Losytskyy MYu, Kovalska VB, Tomachynski SM, Ogul'chansky TYu, Kostenko OM, Kurdyukov VV, Tolmachev OI (2002) Interaction of cyanine dyes with nucleic acids. New (pyrido)(thio)trimethincyanine dye CCyan 40 for fluorescent labeling of oligonucleotides. Biopolymers and Cell 16(4):340-346.
  50. Kovalska VB, Valyukh IV, Lukashov SS, Slominskii YuL, Yarmoluk SM (2002) An investigation of tricarbocyanines "Stains-All" and "iso-Stains-All" as fluorescent nucleic acids probes. Journal of Fluorescence 12(2):209-212.
  51. Yarmoluk SM, Kovalska VB, Kocheshev IO (2002) Influence of the aggregation of homo-N-mer cyanine dyes on the nucleic acids detection sensitivity. Journal of Fluorescence 12(2):155-157.
  52. Valyukh IV, Kovalska VB, Slominskii YL, Yarmoluk SM (2002) Spectroscopic studies of α,γ-disubstituted trimethine cyanine: new fluorescent dye for nucleic acids. Journal of Fluorescence 12(1):105-107.
  53. Yarmoluk SM, Kovalska VB, Kocheshev IO, Kachkovskiy YuYu (2002) Interaction of cyanine dyes with nucleic acids. Simulation of the aggregates structures and their electronic transitions for homo-n-mere benzothiazole cyanine dye using the quantum-mechanical methods, Current Studies of Biotechnology, V.II. – Environment:117-123.
  54. Ogulchansky TYu, Losytskyy MYu, Kovalska VB, Lukashov SS, Yashchuk VM, Yarmoluk SM (2001) Interaction of cyanine dyes with nucleic acids. XVIII. Formation of the carbocyanine dye J-aggregates in nucleic acid grooves. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 57(13):2705-2715.
  55. Yarmoluk SM, Kryvorotenko DV, Balanda AO, Losytskyy MY, Kovalska VB (2001) Proteins and cyanine dyes. Part III. Synthesis and spectroscopic studies of benzothiazolo-4-[1,2,6-trimethylpyridinium] monomethine cyanine dyes for fluorescent detection of bovine serum albumin in solutions. Dyes and Pigments 51(1):41-49.
  56. Yarmoluk SM, Kovalska VB, Kocheshev IA (2001) Interaction of cyanine dyes with nucleic acids. XXVII. Synthesis and spectral properties of novel homodi- and homotrimeric monomethine cyanine dyes. Dyes and Pigments 50(1):21-28.
  57. Ogul'chansky TYu, Losytskyy MYu, Kovalska VB, Yashchuk VM, Yarmoluk SM (2001) Interactions of cyanine dyes with nucleic acids. XXIV. Aggregation of monomethine cyanine dyes in presence of DNA and its manifestation in absorption and fluorescence spectra. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 57(7):1525-1532.
  58. Yarmoluk SM, Kovalska VB, Kryvorotenko DV, Balanda AO, Ogul'chansky TYu (2001) Interaction of cyanine dyes with nucleic acids. XXV. Influence of affinity-modifying groups in the structure of benzothiazol-4-[2,6-dimethylpyridinium] dyes on the spectral properties of the dyes in the presence of nucleic acids.Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 57(7):1533-1540.
  59. Yarmoluk SM, Kryvorotenko DV, Kovalska VB (2001) Interaction of cyanine dyes with nucleic acids. XX. New methods for the preparation of fluorescent probes based on benzothiazol-4-[2,6-dimethylpyridinium] cyanine dyes. Dyes and Pigments 48(3):165-172.
  60. Kryvorotenko DV, Kovalska VB, Yarmoluk SM (2000) The interaction of cyanine dyes with nucleic acids. 16. New fluorescent benzothiazole-4-[2,6-dimethylpyridinium] monomethyne cyanine dyes. Biopolymers and Cell 16(4):320-326.
  61. Kryvorotenko DV, Kovalska VB, Yarmoluk SM (2000) Interaction of cyanine dyes with nucleic acids. 10. Synthesis and spectral properties of novel benzothiazol-4-[2,6-dimethylpyridinium] monomethyne cyanine dyes. Biopolymers and Cell 16(2):145-152.
  62. Losytskyy MYu, Kovalska VB, Yarmoluk SM (2000) Interaction of cyanine dyes with nucleic acids. 9. The study of spectral properties of cyanine dyes-DNA complexes in the presence of organic solvents. Biopolymers and Cell 16(1):75-81.
  63. Kocheshev IA, Kovalska VB, Yarmoluk SM, Akerman B, Matsuka GKh (1999) Interaction of cyanine dyes with nucleic acids. 15. Spectral-fluorescent properties of novel benzothiazolmethylene-2,5-dimethylpyridine homodi- and homotrimere cyanine dyes and their complexes with nucleic acids. Biopolymers and Cell 15(6):557-567.
  64. Kocheshev IA, Kovalska VB, Yarmoluk SM (1999) Interaction of cyanine dyes with nucleic acids. 11. Synthesis and spectral properties of novel 2,6-dimethyl-4-[(3-methyl-1,3-benzothiazol-3-ium-2-yl)methylene]-1,4-dihydro-1-pyridinium homodimer cyanine dyes. Biopolymers and Cell 15(5):449-455.
  65. Yarmoluk SM, Kovalska VB, Lukashov SS, Slominskii YL (1999) Interaction of cyanine dyes with nucleic acids. XII. β-substituted carbocyanines as possible fluorescent probes for nucleic acids detection. Bioorganic and Medicinal Chemistry Letters 9(12):1677-1678.
  66. Yarmoluk SM, Kovalska VB, Kovtun YuP (1999) Interaction of cyanine dyes with nucleic acids. 5. Towards model of “half intercalation” of monomethyne cyanine dyes into double-stranded nucleic acids. Biopolymers and Cell 15(1):75-82.
  67. Yarmoluk SM, Kovalska VB, Smirnova TV, Shandura MP, Kovtun YuP, Matsuka GKh (1996) Interaction of cyanine dyes with nucleic acids. 2. Spectroscopic properties of methyleneoxy analogues of Thiazole Orange.Biopolymers and Cell 12(6):74-81.
  68. Yarmoluk SM, Zhyvoloup AN, Kovalska VB, Klimenko IV, Kukharenko AP, Kovtun YuP, Slominsky YuL (1996) Interaction of cyanine dyes with nucleic acids. 1. Studies on monomethyne as possible fluorescent probes for the nucleic acids. Biopolymers and Cell 12(1):69-75.