Laboratory of Systems Biology

Research Services: 
  • Standard and advanced microarray data pre-processing techniques, including quality control analysis, normalization, batch effect removal, eliminating unspecific probes, performing custom annotation and using custom chip definition (CDF) files for more accurate expression evaluation.
  • Professional analysis of expression data: finding differentially expressed genes with linear models and eBayes methods, gene ontology terms enrichment analysis with modern tools (GSEA, EnrichR), cluster analysis and classification of samples, gene regulatory networks reconstruction (Bayesian inference and information theory approaches), visualization (PCA, heatmaps, boxplots etc) and plots creating with ggplot2 R library.

Laboratory of Bacteriocins Biology

Research Services: 
  • Phage biology (screening, isolation of pure stable genetic lines, identification of phage life strategy);
  • Bacteriocins biology (screening for high-level producers,  developing approaches for high level induction);
  • Phage and bacteriocins concentration and purification facility (ultra-speed and gradient centrifugation, ion-exchange chromatography);
  • Long-term preservation systems for phage products;
  • Identification and analysis of phage virion and genome structure (restrictions mapping, PCR, virion protein range, gross phage morphology via transmission electron microscopy, HPLC);
  • Bioinformatic analysis and annotation of complete phage genomes;
  • Recombinant DNA techniques for target phage proteins.

Laboratory of Computer Modeling

Research Services: 
  • Virtual high-throughput screening using structure-based (molecular docking) and ligand-based (QSAR, pharmacophore modeling) approaches;
  • Identification and analysis of binding sites;
  • Structure-activity relationship (SAR) analysis;
  • Computational studies of drug-receptor interactions;
  • Hit identification and optimization;
  • Hit to lead optimization;
  • In silico ADME predictions;
  • Molecular dynamics of proteins, nucleic acids, extra large heterogeneous systems;
  • Design of target-focused compound libraries with high affinity to the molecular targets (our compound collection contains about 200,000 organic compounds which are immediately available for screening).