Laboratory of Information Technology of Arterial Oscillograms Analysis in Diagnostics and Monitoring of Cardio-Vascular System

Research Services: 
  1. Studying the participation of think tanks (brain activity) in the process of adapting shoulder vessels on the compression sleeve and its dependence on pathological conditions of the brain and heart, immune and metabolic disorders, diseases of the heart and blood vessels in people of all ages and sex at rest and under the influence of the varying impact .
  2. Studying the reaction of the heart in the process of adapting vessels to compress the cuff of the shoulder and its dependence on pathological conditions of the heart, the brain, immune and metabolic disorders, diseases of the heart and blood vessels in people of all ages and sex at rest and under the influence of the varying influence.

Laboratory of Systems Biology

Research Services: 
  • Standard and advanced microarray data pre-processing techniques, including quality control analysis, normalization, batch effect removal, eliminating unspecific probes, performing custom annotation and using custom chip definition (CDF) files for more accurate expression evaluation.
  • Professional analysis of expression data: finding differentially expressed genes with linear models and eBayes methods, gene ontology terms enrichment analysis with modern tools (GSEA, EnrichR), cluster analysis and classification of samples, gene regulatory networks reconstruction (Bayesian inference and information theory approaches), visualization (PCA, heatmaps, boxplots etc) and plots creating with ggplot2 R library.

Environmental Biotechnology Group of the Laboratory of Extremophilic Microorganism Biology

Research Services: 
  • Theoretical calculations for identification optimal microbial process targeted wastewater treatment;
  • Development microbial methods for treatment of multi-component industrial/mining drains containing toxic metal ions, synthetic organic compounds;
  • Development microbial processes targeted reduction the concentration of soluble organics in municipal/industrial wastewaters;
  • Improving efficiency of existing microbial waste treatment process;
  • Microbial processes of food waste recycling (pre –treatment, aerobic/anaerobic digestion);
  • Microbial processes targeted biofuel production from food waste (biogas, biohydrogen, liquid biofuels);
  • Optimization microbial processes of biogas production;

Bioprospecting Group of the Laboratory of Extremophilic Microorganism Biology

Our team has 15-years experience of field works and microbiological sampling in extreme environments with the use of mountain-climbing and speleological sampling techniques:

  • Participation in 7 Antarctic season expeditions (2001-2011);
  • Rock cliffs and coastal area of the Dead Sea, rocks of Negev Desert, Israel (2011);
  • Hypersaline environments of Pomorie, Bulgaria (2012);
  • Highland rainforest, Equador (2013-2014);
  • Karst caves, Ukraine (2015-2016).

The laboratory possesses collection of over 300 bacteria and yeast strains with unique properties isolated from extreme environments.

Research Services: 
  • Microbiological sampling in extreme terrestrial environments – cold, desert, highland, cave;
  • Identification of extreme ecosystem that can be a source of bacterial strains for industrial application;
  • Microbiological counts, isolation and identification of bacteria of C, N, P, Fe, metal cycles;
  • Screening of extreme environments for bacteria, yeast and fungi producing bioactive substances – pigments, enzymes, antibiotics;
  • Isolation of aerobic/anaerobic strains possessing potential industrial application;

Laboratory Protein Biosynthesis

Research Services: 
  • Bioinformatic analysis of a protein of interest.
  • Modelling and analysis of protein structures, molecular docking.
  • Production of the proteins in bacteria and baculovirus systems, site-directed mutagenesis of proteins.
  • Isolation of preparative quantities of fully post-translationally modified oncogenic isoform eEF1A2 of mammalian translation elongation factor and its non-oncogenic paralogue eEF1A1.

Laboratory of Bacteriocins Biology

Research Services: 
  • Phage biology (screening, isolation of pure stable genetic lines, identification of phage life strategy);
  • Bacteriocins biology (screening for high-level producers,  developing approaches for high level induction);
  • Phage and bacteriocins concentration and purification facility (ultra-speed and gradient centrifugation, ion-exchange chromatography);
  • Long-term preservation systems for phage products;
  • Identification and analysis of phage virion and genome structure (restrictions mapping, PCR, virion protein range, gross phage morphology via transmission electron microscopy, HPLC);
  • Bioinformatic analysis and annotation of complete phage genomes;
  • Recombinant DNA techniques for target phage proteins.

Laboratory of Computer Modeling

Research Services: 
  • Virtual high-throughput screening using structure-based (molecular docking) and ligand-based (QSAR, pharmacophore modeling) approaches;
  • Identification and analysis of binding sites;
  • Structure-activity relationship (SAR) analysis;
  • Computational studies of drug-receptor interactions;
  • Hit identification and optimization;
  • Hit to lead optimization;
  • In silico ADME predictions;
  • Molecular dynamics of proteins, nucleic acids, extra large heterogeneous systems;
  • Design of target-focused compound libraries with high affinity to the molecular targets (our compound collection contains about 200,000 organic compounds which are immediately available for screening).