Laboratory of Molecular Genetics

The Department of Molecular Genetics offers services with a wide range of research in the field of plant growing and biotechnology. The aim of the laboratory is to increase the diversity and improvement of agricultural crops, which are of great importance in the agrarian sector of Ukraine and beyond.

Research Services: 
  1. Discovery and application DNA-marker systems for gene or allele identification
  2. Molecular genetic exploration of endangered plants
  3. Identification of genetically modified organisms (GMOs), transgenes, and methods optimization
Area(s) of expertise:

Study the features and mechanisms of structural and functional peculiarities of special genes in crops in vitro and in vivo. Development of molecular markers to select for new high-yield plants with the big content of protein, starch, and some other substances, resistant to pests or tolerance to abiotic stressors, as well as, deployment of methods to identify genetically modified organisms (GMO). Research of genotypic and phenotypic facilities of different crops and their wild relatives (wheat, barley, corn, triticale, spelt, soya, rapeseed, sunflower, potatoes, onion, carrot, tobacco, raspberry, Artemisia, Crambe, Deschampsia, bacteria) can be highly applied in order to receive perspective for agriculture forms of plants.

Selected publications:
  1. Identification of Psy1 genes alleles responsible for carotenoid accumulation in wheat grains
  2. Stepanenko O. V., Stepanenko A. І., Kuzminskiy Ye. V., Morgun B. V.
  3. Biotechnologia Acta. 2017, V. 10, No. 2, P. 57-66, DOI: 10.15407/biotech10.02.057>>>
  4. Connection between ecological plasticity of elite winter wheat varieties and DNA methylation pattern polymorphism within variety
  5. Kravets O. P., Sokolova D. O., Berestyana A. M., Shnurenko O. R., Bannikova M. O., Morgun B. V., Kuchuk M. V., Grodzinsky D. M.
  6. Science and Innovation. 2016, 12(2): 57-67. >>>
  7. SSR analysis in the study of genetic diversity and similarity of barley cultivars
  8. Lakhneko O. R., Morgun B. V., Kalendar R. M., Stepanenko A. I., Troianovska A. V., Rybalka O. I.
  9. Biotechnologia Acta. 2016, V. 9, No. 3, P. 61-68. >>>
  10. Implementation of molecular systems for identification of genetic polymorphism in winter wheat to obtain high-performance specialized varieties
  11. Morgun B. V., Stepanenko A. I., Stepanenko O. V., Bannikova M. O., Holubenko A. V., Nitovska I. O., Maystrov P. D., Grodzinsky D. M.
  12. Science and Innovation. 2016, 12(2): 40-56. >>>
  13. Comparative molecular genetic analysis between Ukrainian and EU registered glyphosate-tolerant rapeseed transgenic plants
  14. Taranenko A. M., Sakhno L. O., Morgun B. V., Kuchuk M. V.
  15. Biotechnologia Acta. 2015, V. 8, No. 2, Р. 52-57. >>>
Specialized labs and equipment:
  1. Electrophoresis of DNA, RNA, protein in agarose or polyacrylamide gels
  2. Polymerase Chain Reaction (PCR) analysis conventional
  3. Polymerase Chain Reaction in real time
  4. Gel documentation photosystem Canon EOS 600D
  5. DNA purification, cloning, sequencing